Madden NFL 2000 (also known as Madden 2000) is a football video game. This was the second of the Madden NFL games to not solely feature John Madden on the cover in North America. The only other one was Madden NFL '95. Most versions of the game cover featured Madden prominently in the foreground, and a recognizable Barry Sanders in a background action graphic. The European PAL edition features only Dorsey Levens on the cover.

The video at the introduction on the CD-ROM versions of the game is an unnamed track by rapper Ludacris. It was the first Madden game to be released for the Game Boy Color. It was also the first Madden game to be released on both PC and Mac, and the first football game ever ported to the Mac.

The Green Bay Packers have the best team overall in the game with a rating of 94, while the worst team is the Cleveland Browns with a rating of 68. For the first time in the series, a limited number of players or their names could be either created or edited as modified rosters